QUANTIFOIL is a perforated support foil with pre-defined hole size, shape and arrangement that has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) when compared with conventional holey film. QUANTIFOIL with circular holes is used in cryo-electron tomographic reconstruction. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. May be preferred over foils with smaller holes if the carbon film should be outside the frame of the image. This option can be desirable in the case of extended objects, such as filamentous objects. Hole size is 3.5 µm. Space between holes is 1 µm. Center to center is 4.5 µm. Grid: Gold. Mesh: 200 .